A simple sensitive HPTLC method developed for the determination of β-sitosterol in the seeds of Mucuna pruriens extract. The stationary phase was precoated silica gel F254. The mobile phase used was ethyl acetate: hexane (6: 4 v/v). The plate was scanned and quantified at 400 nm for β-sitosterol. The proposed HPTLC method provides a faster and cost effective quantitative analysis of β-sitosterol in Mucuna pruriens extracts.
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